Journal
JOURNAL OF PROTEOME RESEARCH
Volume 4, Issue 2, Pages 591-598Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr049769r
Keywords
metabolic profile; metabonomics; C57BL19J mouse; Alpk : ApfCD mouse; nude mouse; chemometrics; UPLC-MS; functional genomics
Categories
Ask authors/readers for more resources
The combination of a new 1.7 mu m reversed-phase packing material, and a chromatographic system, operating at ca. 12 000 psi, (so-called ultra performance liquid chromatography, UPLC) has enabled dramatic increases in chromatographic performance to be obtained for complex mixture separation. This increase in performance is manifested in improved peak resolution, together with increased speed and sensitivity. Here, we show that UPLC offers significant advantages over conventional reversed-phase HPLC amounting to a more than doubling of peak capacity, an almost 10-fold increase in speed and a 3- to 5-fold increase in sensitivity compared to that generated with a conventional 3.5 mu m stationary phase. The first functional genomic application of UPLC-MS technology is illustrated here with respect to multivariate metabolic profiling of urines from males and females of two groups of phenotypically normal mouse strains (C57BL19J and Alpk:ApfCD) and a nude mouse strain. We have also compared this technology to conventional HPLC-MS under similar analytical conditions and show improved phenotypic classification capability of UPLC-MS analysis together with increased ability to probe differential pathway activities between strains as a result of improved analytical sensitivity and resolution.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available