Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 98, Issue 3, Pages 1077-1085Publisher
SPRINGER
DOI: 10.1007/s00253-013-5335-6
Keywords
Pluramycins; Heraclemycins; Heracleum souliei; Traditional Chinese medicine; Endophytes; Anti-BCG
Categories
Funding
- National Program on Key Basic Research Project (973 program) [2013CB734000, 2012CB725200, 2012CB721006]
- Ministry of Science and Technology of China [2013ZX10005004, 2011ZX11102-011-11]
- National Natural Science Foundation of China [81102369, 30911120483, 81102356, 30901849, 30973665, 30911120484]
- CAS Pillar Program [XDA04074000]
- National Distinguished Young Scholar Program in China
- Chinese Academy of Sciences (CAS) [31050110430]
- TWAS
- CAS
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As part of a search for antitubercular substances from natural sources, we screened a library of endophytic microbes (50 strains and 300 crude extracts in total) isolated from traditional Chinese medicines (TCMs) for growth inhibitory activity against Bacillus Calmette-Gu,rin (BCG). The crude extract of Streptomyces sp. strain Y3111, which was associated with the stems of Heracleum souliei, showed good anti-BCG activity with an MIC value of 12.5 mu g/mL. Bioassay-guided isolation led to four new pluramycin-type compounds, heraclemycins A-D (1-4). Their structures were determined by different spectroscopic techniques including HRMSESI, 1D NMR, and 2D NMR. This is the first report of pluramycin analogues produced by TCM endophytic microbes as well as the first example of BCG-selective pluramycins. Heraclemycin C (3) showed selective antitubercular activity against BCG with a MIC value of 6.25 mu g/mL and a potential new mode of action.
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