Journal
STEM CELLS
Volume 23, Issue 3, Pages 299-305Publisher
WILEY
DOI: 10.1634/stemcells.2004-0252
Keywords
human embryonic stem cells; RNA interference; transgene expression retroviral and lentiviral vectors; Oct4; Nanog; self-renewal
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Funding
- NHLBI NIH HHS [HL71625] Funding Source: Medline
- NIDDK NIH HHS [DK59279] Funding Source: Medline
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RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
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