Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 97, Issue 20, Pages 8987-8995Publisher
SPRINGER
DOI: 10.1007/s00253-013-4728-x
Keywords
Lysinibacillus fusiformis; Oleate hydratase; Ricinoleic acid; Dihydroxystearic acid production; Emulsifying activity
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Funding
- Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries [112002-3]
- Korea Healthcare Technology R&D Project, Ministry for Health & Welfare, Republic of Korea [2012-009]
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A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction conditions for the production of 10,12-dihydroxystearic acid were optimized as follows: pH 6.5, 30 A degrees C, 15 g l(-1) ricinoleic acid, 9 mg ml(-1) of enzyme, and 4 % (v/v) methanol. Under the optimized conditions, the enzyme produced 13.5 g l(-1) 10,12-dihydroxystearic acid without detectable byproducts in 3 h, with a conversion of substrate to product of 90 % (w/w) and a productivity of 4.5 g l(-1) h(-1). The emulsifying activity of 10,12-dihydroxystearic acid was higher than that of oleic acid, ricinoleic acid, stearic acid, and 10-hydroxystearic acid, indicating that 10,12-dihydroxystearic acid can be used as a biosurfactant.
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