Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 95, Issue 6, Pages 1479-1489Publisher
SPRINGER
DOI: 10.1007/s00253-011-3823-0
Keywords
Biocatalysis; Baeyer-Villiger monooxygenase; Sulfoxidation; Enantioselectivity
Categories
Funding
- Integrated Biosynthesis Organic Synthesis (IBOS) program of the Netherlands Organisation for Scientific Research (NWO)
- EU-FP7 Oxygreen project
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To expand the available set of Baeyer-Villiger monooxygenases (BVMOs), we have created expression constructs for producing 22 Type I BVMOs that are present in the genome of Rhodococcus jostii RHA1. Each BVMO has been probed with a large panel of potential substrates. Except for testing their substrate acceptance, also the enantioselectivity of some selected BVMOs was studied. The results provide insight into the biocatalytic potential of this collection of BVMOs and expand the biocatalytic repertoire known for BVMOs. This study also sheds light on the catalytic capacity of this large set of BVMOs that is present in this specific actinomycete. Furthermore, a comparative sequence analysis revealed a new BVMO-typifying sequence motif. This motif represents a useful tool for effective future genome mining efforts.
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