Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 96, Issue 1, Pages 113-121Publisher
SPRINGER
DOI: 10.1007/s00253-011-3803-4
Keywords
Streptomyces coelicolor; DNA affinity capture assay; gamma-Butyrolactone; scbR/A promoter; ScbR; Antibiotic production
Categories
Funding
- National Research Foundation [2010-0009942, 2009-0094021]
- Korea Government (MEST)
- Korea Ministry of Environment [201-101-007]
- National Research Foundation of Korea [2009-0094021, 2010-0009942] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
gamma-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. gamma-Butyrolactone receptors are considered important regulatory proteins, and various gamma-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (gamma-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known gamma-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of gamma-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like gamma-butyrolactone binding regulator (SlbR) in S. coelicolor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
