Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 94, Issue 6, Pages 1469-1482Publisher
SPRINGER
DOI: 10.1007/s00253-012-4095-z
Keywords
Growth model; Monod model; Recombinant protein production; Process analytical technology (PAT)
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Funding
- Science Foundation of Ireland [08/IN.1/B1948]
- Science Foundation Ireland (SFI) [08/IN.1/B1948] Funding Source: Science Foundation Ireland (SFI)
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Understanding the growth characteristics of microorganisms is an essential step in bioprocessing, not only because product formation may be growth-associated but also because they might influence cell physiology and thereby product quality. The specific growth rate, a key variable of many bioprocesses, cannot be measured directly and relies on the estimation through other measurable variables such as biomass, substrate, or product concentrations. Techniques for real-time estimation of the specific growth rate in microbial fed-batch cultures are discussed in the present paper. The advantages and limitations of different models and various monitoring techniques are discussed, highlighting the importance of the specific growth rate in the development of fast, reliable, and robust processes for the production of high-value products such as recombinant proteins.
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