Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 93, Issue 5, Pages 2233-2239Publisher
SPRINGER
DOI: 10.1007/s00253-012-3882-x
Keywords
Oligoalginate lyase; Alg17C; Polysaccharide lyase-17; Exo-type alginate lyase; Saccharophagus degradans 2-40
Categories
Funding
- Ministry for Food, Agriculture, Forestry and Fisheries, Korea
- Pioneer Research Center Program [2011-0002327]
- Ministry of Education, Science and Technology, South Korea
- Institute of Biomedical Sciences and Food Safety, Korea University
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Macroalgae are considered to be promising biomass for fuels and chemicals production. To utilize brown macroalgae as biomass, the degradation of alginate, which is the main carbohydrate of brown macroalgae, into monomeric units is a critical prerequisite step. Saccharophagus degradans 2-40 is capable of degrading more than ten different polysaccharides including alginate, and its genome sequence demonstrated that this bacterium contains several putative alginate lyase genes including alg17C. The gene for Alg17C, which is classified into the PL-17 family, was cloned and overexpressed in Escherichia coli. The recombinant Alg17C was found to preferentially act on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-l-erythro-5-hexoseulose uronic acid. The optimal pH and temperature for Alg17C were found to be 6 and 40 A degrees C, respectively. The K (M) and V (max) of Alg17C were 35.2 mg/ml and 41.7 U/mg, respectively. Based on the results of this study, Alg17C could be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production.
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