Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 11, Issue 6, Pages 1718-1724Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200400537
Keywords
conjugated polymers; DNA recognition; electrostatic interactions; proteins; sensors
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Single-stranded DNA (ss-DNA) can specifically bind to various targets, including a complementary ss-DNA, ions, proteins, drugs, and so forth. When binding takes place, the oligonucleotide probe often undergoes a conformational transition. This conformational change of the negatively charged ss-DNA can be detected by using a water-soluble, cationic polythiophene derivative, which transduces the complex formation into an optical (colorimetric or fluorometric) signal without any labeling of the probe or the target. This simple and rapid methodology has enabled the specific and sensitive detection of nucleic acids and human thrombin. This new biophotonic tool can easily be applied to the detection of various other biomolecules and is also useful in the high-throughput screening of new drugs.
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