Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 97, Issue 3, Pages 1103-1111Publisher
SPRINGER
DOI: 10.1007/s00253-012-4399-z
Keywords
Sophorolipids; Candida bombicola; Volumetric productivity; High cell density fermentation; Biosurfactants
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Funding
- Sino-Danish Scientific and Technological Cooperation project
- Danish government [08-039647]
- Danida Fellowship programme
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To achieve high time-space efficiency for sophorolipid production with yeast Candida bombicola, a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal cell density and (2) the computer-aided adjustment of physical parameters and the controlled feeding of substrates for enhanced volumetric productivity. Both stages have been successfully implemented in a 10-L fermenter, where up to 80 g dry cell weight/L was obtained and a remarkably high volumetric productivity (> 200 g isolated sophorolipids/L/day) was achieved. Both the biomass and volumetric productivity were markedly higher than previously reported. Specifically, the high productivity of sophorolipids could be attained on a very short time scale (24 h), highlighting the industrial potential of the platform developed in this work.
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