Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 97, Issue 4, Pages 1501-1511Publisher
SPRINGER
DOI: 10.1007/s00253-012-4358-8
Keywords
Phenolic acid decarboxylase; Bioconversion; p-Coumaric acid; p-Hydroxystyrene; Biphasic reaction; 1-Octanol
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Funding
- World Class University program through the National Research Foundation of Korea
- Ministry of Education, Science and Technology [R322009000102130]
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Phenolic acid decarboxylase (PAD) catalyzes the non-oxidative decarboxylation of p-coumaric acid (pCA) to p-hydroxystyrene (pHS). PAD from Bacillus amyloliquefaciens (BAPAD), which showed k (cat)/K (m) value for pCA (9.3 x 10(3) mM(-1) s(-1)), was found as the most active one using the Subgrouping Automata program and by comparing enzyme activity. However, the production of pHS of recombinant Escherichia coli harboring BAPAD showed only a 22.7 % conversion yield due to product inhibition. Based on the partition coefficient of pHS and biocompatibility of the cell, 1-octanol was selected for the biphasic reaction. The conversion yield increased up to 98.0 % and 0.83 g/h/g DCW productivity was achieved at 100 mM pCA using equal volume of 1-octanol as an organic solvent. In the optimized biphasic reactor, using a three volume ratio of 1-octanol to phosphate buffer phase (50 mM, pH 7.0), the recombinant E. coli produced pHS with a 88.7 % conversion yield and 1.34 g/h/g DCW productivity at 300 mM pCA.
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