Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1067, Issue 1-2, Pages 73-83Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.10.107
Keywords
proteome; proteomics; NanoLC-MS/MS
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Current nano-scale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) approaches in proteome research are reviewed from an analytical perspective. For comprehensive analysis of cellular proteins, analytical methods with higher resolution, sensitivity, and wider dynamic range are required. Miniaturized LC coupled with tandem mass spectrometry is currently one of the most versatile techniques. In this review, the current status of nanoLC-MS/MS systems as well as data management systems is addressed. In addition, the future prospects for complete proteomics are discussed. (c) 2004 Elsevier B.V. All rights reserved.
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