Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 93, Issue 5, Pages 1917-1925Publisher
SPRINGER
DOI: 10.1007/s00253-011-3718-0
Keywords
Polylactide; Biobased plastic; PHA synthase; Polyhydroxyalkanoate; Polyhydroxybutyrate
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Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan [23310059, B01]
- Grants-in-Aid for Scientific Research [23310059] Funding Source: KAKEN
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The first biosynthetic system for lactate (LA)-based polyesters was previously created in recombinant Escherichia coli (Taguchi et al. 2008). Here, we have begun efforts to upgrade the prototype polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C. glutamicum to generate monomer substrates, lactyl-CoA (LA-CoA), and 3-hydroxybutyryl-CoA (3HB-CoA), for the copolymerization catalyzed by the LA-polymerizing enzyme (LPE). LA-CoA was synthesized by D-lactate dehydrogenase and propionyl-CoA transferase, while 3HB-CoA was supplied by beta-ketothiolase (PhaA) and NADPH-dependent acetoacetyl-CoA reductase (PhaB). The functional expression of these enzymes led to a production of P(LA-co-3HB) with high LA fractions (96.8 mol%). The omission of PhaA and PhaB from this pathway led to a further increase in LA fraction up to 99.3 mol%. The newly engineered C. glutamicum potentially serves as a food-grade and biomedically applicable platform for the production of poly(lactic acid)-like polyester.
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