Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 93, Issue 4, Pages 1769-1776Publisher
SPRINGER
DOI: 10.1007/s00253-011-3508-8
Keywords
Anaerobic; Degradation; Electron transfer; Methyl orange (MO); Mutant strains; Mtr respiratory pathway; Riboflavin; Shewanella oneidensis
Categories
Funding
- National Natural Science Foundation of China [20907050]
- Outstanding Young Scientists Foundation of Anhui Province, China [10040606Y27]
Ask authors/readers for more resources
In this work, we investigated the anaerobic decolorization of methyl orange (MO), a typical azo dye, by Shewanella oneidensis MR-1, which can use various organic and inorganic substances as its electron acceptor in natural and engineered environments. S. oneidensis MR-1 was found to be able to obtain energy for growth through anaerobic respiration accompanied with dissimilatory azo-reduction of MO. Chemical analysis shows that MO reduction occurred via the cleavage of azo bond. Block of Mtr respiratory pathway, a transmembrane electron transport chain, resulted in a reduction of decolorization rate by 80%, compared to the wild type. Knockout of cymA resulted in a substantial loss of its azo-reduction ability, indicating that CymA is a key c-type cytochrome in the electron transfer chain to MO. Thus, the MtrA-MtrB-MtrC respiratory pathway is proposed to be mainly responsible for the anaerobic decolorization of azo dyes such as MO by S. oneidensis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available