Journal
CANCER LETTERS
Volume 219, Issue 2, Pages 205-213Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2004.07.027
Keywords
cholangiocarcinoma; matrix metalloproteinase; tumor necrosis factor-alpha; invasion
Categories
Ask authors/readers for more resources
We studied the effect of TNF-alpha stimulation on a cholangiocarcinoma cell line, CCKS1. CCKS1 expressed only one type TNF receptor,TNFR2. Treatment of CCKS1 with TNF-a substantially activated NF kappa B, MAPK and Akt signalings which in turn activated matrix metalloproteinase-9 (MMP-9) secretion and in vitro invasiveness of CCKS1. Pretreatment of cells with anti-TNFR2 neutralizing antibody inhibited the TNF-alpha-dependent signaling and MMP-9 secretion and subsequently blocked invasion in vitro. Moreover, an inhibitor for matrix metalloproteinase, Galardin, suppressed the invasion in a dose-dependent manner. Similarly, pharmacological inhibition of signaling clearly suppressed the TNF-alpha dependent MMP-9 secretion. These results strongly suggest that TNF-alpha-TNFR2 signaling plays an important role to convert the cholangiocarcinoma cells to be more aggressive one. (c) 2004 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available