Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 114, Issue 1, Pages 80-87Publisher
WILEY
DOI: 10.1002/ijc.20669
Keywords
lung metastasis; CT26; NKT cells; IL-13 inhibitor; immunosurveillance
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We have previously observed a novel role of natural killer T (NKT) cells in negative regulation of antitumor immune responses against an immunogenic regressor tumor expressing a transfected viral antigen. Here, we investigated whether hidden spontaneous antitumor immunosurveillance, in the absence of a vaccine, could be revealed by disruption of this negative regulatory pathway involving CD4(+) NKT cells and interieukin-13 (IL-13), in a murine pulmonary metastasis model of a nontransfected, nonregressor, syngeneic tumor, the CT26 colon carcinoma. Lung metastases of CT26 were decreased in CD4(+) T cell-depleted BALB/c mice, suggesting that CD4(+) T cells were involved in negative regulation of antitumor responses. CD1-knock out (CD1-KO) mice, which have conventional CD4(+) T cells and CD4(+)CD25(+) regulatory T cells but lack CD1-restricted CD4(+) NKT cells, were significantly resistant to lung metastasis of CT26. The metastases were not further decreased in CD4(+) T cell-depleted CDl-KO mice, implying that CD4(+) NKT cells might be the primary negative regulator of antitumor immune responses in BALB/c mice. CD8(+) T cells were found to act as effectors in antitumor immune responses, since the inhibition of lung metastases observed in naive CD1-KO or CD4(+) T cell-depleted mice was abrogated by depletion of CD8(+) T cells. Lung metastases were significantly decreased by treatment of mice with an IL-13 inhibitor, but not by deficiency or inhibition of IL-4. Thus, even for a nonregressor tumor, immunosurveillance exists but is negatively regulated via CD4(+) NKT cells possibly mediated by IL-13, and can be unmasked by removal of these negative regulatory components. Published 2004 Wiley-Liss, Inc.
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