4.8 Article

Chromosomal translocations in yeast induced by low levels of DNA polymerase: A model for chromosome fragile sites

Journal

CELL
Volume 120, Issue 5, Pages 587-598

Publisher

CELL PRESS
DOI: 10.1016/j.cell.2004.12.039

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Funding

  1. NCI NIH HHS [CA09156] Funding Source: Medline
  2. NIGMS NIH HHS [GM52319] Funding Source: Medline

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In the yeast Saccharomyces cerevisiae, reduced levels of the replicative a DNA polymerase result in greatly elevated frequencies of chromosome translocations and chromosome loss. We selected translocations in a small region of chromosome III and found that they involve homologous recombination events between yeast retrotransposons (Ty elements) on chromosome III and retrotransposons located on other chromosomes. One of the two preferred sites of these translocations on chromosome III involve two Ty elements arrayed head-to-head; disruption of this site substantially reduces the rate of translocations. We demonstrate that this pair of T elements constitutes a preferred site for double-strand DNA breaks when DNA replication is compromised, analogous to the fragile sites observed in mammalian chromosomes.

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