Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 87, Issue 5, Pages 1917-1925Publisher
SPRINGER
DOI: 10.1007/s00253-010-2692-2
Keywords
Pathogenic bacteria; X-ray diffraction pattern (XRD); PL spectroscopy; FTIR spectroscopy; ZnO nanoparticles and antibacterial activity
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Funding
- KOSEF (Korea Science and Engineering Foundation) [R01-2007-000-20810-0]
- Ministry of Knowledge Economy (MKE), Republic of Korea [C0008851] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2007-0056050] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The antibacterial activity of ZnO nanoparticles has been investigated and presented in this paper. Nanoparticles were prepared via non-hydrolytic solution process using zinc acetate di-hydrate (Zn(CH(3)COO)(2)center dot 2H(2)O) and aniline (C(6)H(5)NH(2)) in 6 h refluxing at similar to 65 A degrees C. In the presence of four pathogens such as Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Klebsiella pneumoniae, the antibacterial study of zinc oxide nanoparticles were observed. The antibacterial activity of ZnO nanoparticles (ZnO-NPs) were studied by spectroscopic method taking different concentrations (5-45 mu g/ml) of ZnO-NPs. Our investigation reveals that the lowest concentration of ZnO-NPs solution inhibiting the growth of microbial strain is found to be 5 mu g/ml for K. pneumoniae, whereas for E. coli, S. aureus, and S. typhimurium, it was calculated to be 15 mu g/ml. The diameter of each ZnO-NPs lies between 20 and 30 nm as observed from FESEM and transmission electron microscopy images. The composition of synthesized material was analyzed by the Fourier transform infrared spectroscopy, and it shows the band of ZnO at 441 cm(-1). Additionally, on the basis of morphological and chemical observations, the chemical reaction mechanism of ZnO-NPs was also proposed.
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