Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 89, Issue 4, Pages 1009-1017Publisher
SPRINGER
DOI: 10.1007/s00253-010-2904-9
Keywords
D-Lactate; Sporolactobacillus sp.; Peanut meal; Simultaneous enzymatic hydrolysis
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Funding
- National Basic Research Program of China [2007CB707803]
- Chinese National Programs for High Technology Research and Development [2006AA020102, 2007AA10Z360]
- National Natural Science Foundation of China [30900022]
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Highly efficient d-lactate production by Sporolactobacillus sp. strain CASD was demonstrated in this study. Peanut meal was found to be a better nutrient than yeast extract, soybean meal, soybean peptone, corn steep, liquor beef extract, and ammonium sulfate in the production of d-lactate. To improve the utilization of peanut meal, the material was enzymatically hydrolyzed and simultaneously utilized as the nitrogen source in d-lactate fermentation. Very high d-lactate production (207 g/L) was obtained using 40 g/L of peanut meal in 30-L fed-batch fermentation, with the average productivity of 3.8 g/(L center dot h) and optical purity of 99.3%. The production of such a high concentration of optically pure d-lactate by strain CASD, with the simultaneous enzymatic hydrolysis of peanut meal and fermentation, represents a new cost-efficient and integrated method for d-lactate production using agricultural by-products.
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