Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 89, Issue 4, Pages 1119-1125Publisher
SPRINGER
DOI: 10.1007/s00253-010-2940-5
Keywords
2,3-Butanediol; Klebsiella oxytoca; Glucose; Xylose; Carbon catabolite repression; Mutant cAMP receptor protein
Categories
Funding
- National Natural Science Foundation of China [20606018, 21006049, 20936002]
- National Basic Research Program of China [2007CB707805, 2009CB724700, 2011CB200906]
- Ministry of Education of China [NCET-09-0157]
- Nanjing University of Technology [BSCX200808]
- China Postdoctoral Science Foundation [20100471328]
- Jiangsu Planned Projects for Postdoctoral Research Funds of China [1001015 C]
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Microbial preference for glucose implies incomplete and/or slow utilization of lignocellulose hydrolysates, which is caused by the regulatory mechanism named carbon catabolite repression (CCR). In this study, a 2,3-butanediol (2,3-BD) producing Klebsiella oxytoca strain was engineered to eliminate glucose repression of xylose utilization. The crp(in) gene, encoding the mutant cyclic adenosine monophosphate (cAMP) receptor protein CRP(in), which does not require cAMP for functioning, was characterized and overexpressed in K. oxytoca. The engineered recombinant could utilize a mixture of glucose and xylose simultaneously, without CCR. The profiles of sugar consumption and 2,3-BD production by the engineered recombinant, in glucose and xylose mixtures, were examined and showed that glucose and xylose could be consumed simultaneously to produce 2,3-BD. This study offers a metabolic engineering strategy to achieve highly efficient utilization of sugar mixtures derived from the lignocellulosic biomass for the production of bio-based chemicals using enteric bacteria.
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