Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 86, Issue 5, Pages 1585-1592Publisher
SPRINGER
DOI: 10.1007/s00253-010-2460-3
Keywords
s-Triazine herbicides; Atrazine; Cyanuric acid; s-Triazine ring mineralization; Gram-positive strains; Nocardioides
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A novel s-triazine-mineralizing bacterium-Nocardioides sp. strain DN36-was isolated from paddy field soil treated with ring-U-C-14-labeled simetryn ([C-14]simetryn) in a model paddy ecosystem (microcosm). In a tenfold-diluted R2A medium, strain DN36 liberated (CO2)-C-14 from not only [C-14]simetryn but also three ring-U-C-14-labeled s-triazines: atrazine, simazine, and propazine. We found that DN36 mineralized ring-U-C-14-cyanuric acid added as an initial substrate, indicating that the bacterium mineralized s-triazine herbicides via a common metabolite, namely, cyanuric acid. Strain DN36 harbored a set of genes encoding previously reported s-triazine-degrading enzymes (TrzN-AtzB-AtzC), and it also transformed ametryn, prometryn, dimethametryn, atraton, simeton, and prometon. The findings suggest that strain DN36 can mineralize a diverse range of s-triazine herbicides. To our knowledge, strain DN36 is the first Nocardioides strain that can individually mineralize s-triazine herbicides via the ring cleavage of cyanuric acid. Further, DN36 could not grow on cyanuric acid, and the degradation seemed to occur cometabolically.
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