Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 89, Issue 1, Pages 57-62Publisher
SPRINGER
DOI: 10.1007/s00253-010-2853-3
Keywords
Escherichia coli; 1,3-Propanediol; Glucose; Metabolic engineering
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Funding
- National Natural Science Foundation of China [30870022]
- National Basic Research Program of China [2011CB707405]
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1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive synthesis. The aim of this study was to engineer an Escherichia coli strain that can produce 1,3-propanediol directly from glucose. We successfully constructed a stress-induced metabolic pathway from glucose to 1,3-propanediol in recombinant E. coli by the expression of gpd1 and gpp2 genes from Saccharomyces cerevisiae and dha operon from Klebsiella pneumoniae, respectively. Batch cultivation of the recombinant E. coli showed that 12.1 g/L 1,3-propanediol was accumulated in the culture without using any inducer.
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