Journal
VACCINE
Volume 23, Issue 16, Pages 1949-1956Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2004.10.012
Keywords
HIV-1; subtype AE; DNA; Fowlpoxvirus; macaque
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Funding
- NIAID NIH HHS [N01-AI-05395] Funding Source: Medline
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To induce broad T cell immunity to HIV- 1, we evaluated the safety, immunogenicity and dose-response relationship of DNA and recombinant Fowlpoxvirus (rFPV) vaccines encoding five shared HIV subtype AE genes (Gag, Pol, Env, Tat, Rev) in pigtail macaques. The DNA (three doses of either 1 mg or 4.5 mg) and rFPV (a single boost of either 5 x 107 or 2 x 10(8) plaque forming units) vaccines were administered intramuscularly without adjuvants. Broadly reactive HIV-specific T cell immunity was stimulated by all doses of the vaccines administered, without significant differences between the high and low doses studied. The vaccines induced both CD4 and CD8 T cell responses to Gag, Pol, Env and Tat/Rev proteins, with CD4 T cell responses being greater in magnitude than CD8 T cell responses. The vaccine-induced T cell responses had significant cross-recognition of heterologous HIV-1 proteins from non-AE HIV-1 subtypes. In conclusion, these subtype AE HIV-1 DNA and rFPV vaccines were safe, induced broad T-cell immunity in macaques, and are suitable for progression into clinical trials. (c) 2004 Elsevier Ltd. All rights reserved.
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