Journal
FEBS LETTERS
Volume 579, Issue 7, Pages 1625-1632Publisher
WILEY
DOI: 10.1016/j.febslet.2005.01.077
Keywords
heart mitochondria; potassium channel; ATP; magnesium; black lipid membrane
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Mitochondrial ATP-regulated potassium (mitoKATP) channels play an important role in cardioprotection. Single channel activity was measured after reconstitution of inner mitochondrial membranes from bovine myocardium into a planar lipid bilayer. After incorporation, the potassium channel was recorded with a mean conductance of 103 +/- 9 pS. The channel activity was inhibited by ATP/Mg and activated by GDP. Magnesium ions alone affected, in a dose dependent manner, both the channel conductance and the open probability. Magnesium ions regulated the mitoKATP channel only when added to the trans compartment. We conclude that Mg2+ regulates the cardiac mitoKATP channel from the matrix site by affecting both the channel conductance and gating. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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