Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 80, Issue 4, Pages 709-717Publisher
SPRINGER
DOI: 10.1007/s00253-008-1574-3
Keywords
Streptomyces coelicolor; DNA chip; clustering; standard deviation; pathway-specific regulator
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Funding
- Korea Science and Engineering Foundation (KOSEF)
- Korea Government (MOST) [R01-2006-000-10234-0]
- Korea Research Foundation, Republic of Korea [KRF-2005-005-J16002]
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In order to identify the regulators involved in antibiotic production or time-specific cellular events, the messenger ribonucleic acid (mRNA) expression data of the two gene clusters, actinorhodin (ACT) and undecylprodigiosin (RED) biosynthetic genes, were clustered with known mRNA expression data of regulators from S. coelicolor using a filtering method based on standard deviation and clustering analysis. The result identified five regulators including two well-known regulators namely, SCO3579 (WlbA) and SCO6722 (SsgD). Using overexpression and deletion of the regulator genes, we were able to identify two regulators, i.e., SCO0608 and SCO6808, playing roles as repressors in antibiotics production and sporulation. This approach can be easily applied to mapping out new regulators related to any interesting target gene clusters showing characteristic expression patterns. The result can also be used to provide insightful information on the selection rules among a large number of regulators.
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