Journal
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
Volume 85, Issue 3, Pages 163-175Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/03067310412331334844
Keywords
pesticides; amperometry; inhibition biosensor; acctylcholinesterase; nanoparticles ZrO2; chitosan
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An amperometric pesticides inhibition biosensor has been developed and used for determination of pesticides in vegetable samples. To eliminate the interference of ascorbic acid. multilayer films of polyelectrolyte (chitosan/polystyrensulfonate) were coated on the glass carbon electrode. Then, acetylcholinesterase was immobilized on the electrode based on surface-treatcd nanoporous ZrO2,/chitosan composite film as immobilization matrix. As a modified substrate, acetylthiocholine was hydrolysed by acetylcholinesterase and produced thiocholine which can be oxidized at +700mV vs. SCE. Pesticides inhibit the activity of enzyme with an effect of decreasing of oxidation current. The experimental conditions were optimized. The electrode has a linear response to acetylthiocholine within 9.90 X 10(-6) to 2.03 x 10(-3) M. The electrode provided a linear response over a concentration range of 6.6 x 10(-6) to 4.4 x 10(-4) M for phoxim with a detection limit of 1.3 x 10(-6) M, over a range of 1.0 x 10(-8) to 5.9 x 10(-7) M for malathion, and over a range of 8.6 x 10(-6) to 5.2 x 10(-4) M for dimethoate. This biosensor has been used to determine pesticides in a real vegetable sample.
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