Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 11, Issue 7, Pages 1981-1988Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200400960
Keywords
DNA; microarrays; monolayers; oligonucleotides; phosphonates
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A new process for preparing, oligonucleotide arrays is described that uses surface grafting chemistry which is fundamentally different from the electrostatic adsorption and organic covalent binding methods normally employed. Solid supports are modified with a mixed organic/inorganic zirconium phosphonate monolayer film providing a stable, well-defined interface. Oligonucleotide probes terminated with phosphate are spotted directly on to the zirconated surface forming a covalent linkage. Specific binding of terminal phosphate groups with minimal binding of the internal phosphate diesters has been demonstrated. ne mixed organic/inorganic thin films have also been extended for use arraying DNA duplex probes, and therefore represent a viable general approach to DNA-based bioarrays. Ideas for interfacing mixed organic/inorganic interfaces to other bioapplications are also discussed.
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