Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 79, Issue 3, Pages 363-370Publisher
SPRINGER
DOI: 10.1007/s00253-008-1442-1
Keywords
luxCDABE; biosensor; mercury; luminescence
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Two Hg(2+)-specific biosensors were constructed using bacterial luciferase as reporter gene and plasmid-free Pseudomonas putida X4 and Enterobacter aerogenes NTG-01 as host strains. The performance of X4 biosensor was compared with that of NTG-01 biosensor in the same assay conditions. The maximum bioluminescence for X4 (pmerRluxCDABE-Kan) biosensor was found during the midexponential phase and that for NTG-01 (pmerRluxCDABE-Kan) was at the late exponential phase. The shortest induction time of two biosensors was 30 min. The maximum light signal output for NTG-01 and X4 sensors was observed at the incubation time of 5 and 4 h, respectively. The lowest detectable concentration of mercury by the two biosensors were both of 100 pM at 28 degrees C, pH 7 and an initial cell number of 10(6) CFU ml(-1). Cd(2+), Zn(2+), Co(2+), Cu(2+), and Pb(2 +) ions at nanomolar level did not interfere with the measurement by the biosensors. These results show that the sensitivity of the two biosensors is sufficient for the detection of Hg(2+) under most contaminated environments.
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