Journal
JOURNAL OF CONTROLLED RELEASE
Volume 103, Issue 2, Pages 405-418Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2004.12.018
Keywords
pH-sensitive micelles; polyHis-b-PEG; folate; multidrug resistance
Funding
- NCI NIH HHS [CA101850] Funding Source: Medline
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In order to overcome multidrug resistance in solid tumors, doxorubicin (DOX) loaded pH-sensitive micelles of which surface was decorated with folate (PHSM/f) were evaluated both in vitro and in vivo experiments. PHSM/f were fabricated from a mixture of two block copolymers of poly(L-histidine) (M-n: 5K)-b-PEG (M-n: 2K)-folate (polyHis/PEG-folate) (75 wt.%) and poly(L-lactic acid) (M-n: 3K)-b-PEG (M-n: 2K)-folate (PLLA/PEG-folate) (25 wt.%). The PHSM/f showed more than 90% cytotoxicity of DOX resistant MCF-7 (MCF-7/DOXR) when cultured with PHSM/f at a concentration of 10 mu g/ml DOX. The result was interpreted by a sequential event of active internalization of PHSM/f via folate-receptor mediated endocytosis and ionization of His residues which result in micelle destabilization and probably disturbance of endosomal membranes. This potential mechanism may endow the drug carriers to bypass Pgp efflux pump and sequestration of DOX in acidic intracellular compartments, yielding high cytotyoxicity. Experimental evaluation of tumor regression was carried out in a small animal model bearing s.c. MCF-7 or MCF-7/DOXR xenografts. The tumor (MCF-7/DOXR) volumes of mice treated with PHSM/f were significantly less than control groups treated with free DOX or similar micelles but without folate (PHSM). In the MCF-7/DOXR xenograft model, the accumulated DOX level of PHSM/f in solid tumors was 20 times higher than free DOX group, and 3 times higher than PHSM group. The results demonstrate that PHSM/f is a viable means for treating drug resistant tumors. (c) 2005 Elsevier B.V. All rights reserved.
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