Journal
JOURNAL OF VIROLOGY
Volume 79, Issue 7, Pages 4506-4509Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.79.7.4506-4509.2005
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Funding
- NCI NIH HHS [CA68051, R01 CA068051] Funding Source: Medline
- NIDCR NIH HHS [P01 DE014388, DE14388] Funding Source: Medline
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The Epstein-Barr virus (EBV) nuclear antigen 3B (EBNA-3B) is considered nonessential for EBV-mediated B-cell growth transformation in vitro based on three virus isolates with EBNA-3B mutations. Two of these isolates could potentially express truncated EBNA-3B products, and, similarly, we now show that the third isolate, IB4, has a point mutation and in-frame deletion of 263 amino acids. In order to test whether a virus with EBNA-3B completely deleted can immortalize B-cell growth, we first cloned the EBV genome as a bacterial artificial chromosome (BAC) and showed that the BAC-derived virus was B-cell immortalization competent. Deletion of the entire EBNA-3B open reading frame from the EBV BAC had no adverse impact on growth of EBV-immortalized B cells, providing formal proof that EBNA-3B is not essential for EBV-mediated B-cell growth transformation in vitro.
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