Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 162, Issue 4, Pages 421-428Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2004.07.017
Keywords
benzofuran isophthalate; fluorescence ratio microscopy; quince; salinity stress; sodium uptake; sugar beet; wheat
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The uptake of sodium into protoplasts of quince (Cydonia oblonga Mitt, clone BA29), sugar beet (Beta vulgaris L. cv. Monohill), and wheat (Triticum aestivum L. cv. Kadett) was determined by use of the acetoxy methyl ester of the fluorescent sodium-binding benzofuran isopthalate (SBFI-AM). In the presence of 1 mM CaCl2, little sodium was taken up in the cytosol. of quince mesophyll cells compared to cytosols of sugar beet and wheat. Upon addition of 40 mM NaCl, approximately the same amount of sodium was taken up in leaf and root protoplasts of wheat, but no sodium was taken up in quince. However, in calcium-free medium, obtained by addition of ethylene glycol tetra acetic acid (EGTA), quince protoplasts transiently took up sodium in the cytosol when 200-400 mM NaCl was added to the protoplast medium. Moreover, after cultivation of quince in the presence of 200 mM sodium for 4 weeks, the cytosol of isolated protoplasts did not take up any sodium at all from a calcium-free medium. The results show that protoplasts from salt tolerant quince only temporarily take up sodium in the cytosol and that they have a mechanism for fast extrusion of sodium from that compartment. These mechanisms are probably important for the high salt tolerance of quince. Calcium blocks the sodium uptake into the cytosol. of both quince and wheat protoplasts. (c) 2004 Elsevier GmbH. All rights reserved.
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