Journal
CURRENT OPINION IN PLANT BIOLOGY
Volume 8, Issue 2, Pages 211-215Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/j.pbi.2005.01.004
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TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful reverse genetic technique that employs a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms. Its advantages over other reverse genetic techniques include its applicability to virtually any organism, its facility for high-throughput and its independence of genome size, reproductive system or generation time. TILLING is currently being used for the detection of both induced and natural variation in several plant species.
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