Journal
CRYOBIOLOGY
Volume 50, Issue 2, Pages 216-222Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2005.01.003
Keywords
vitrification; centrifugation; hypertonic condition; lipid droplets; GV oocytes; pig
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In the present study, we examined a novel lipid removal method, centrifugation in solutions made hypertonic by adding 0.27 M Sugar. This allowed the lipid to be extruded and removed without the loss of active mitochondria or extra cytoplasm. The type of sugar influenced the proportion of oocytes that could be stratified by centrifugation. Glucose induced the highest extrusion rate of lipid droplets. After vitrification the rates of survival, germinal vesicle breakdown and metaphase II were 30, 26, and 7%, respectively, for lipid-removed GV oocytes; this was significantly higher (P < 0.05) than for corresponding vitrified lipid-intact oocytes (2, 0, and 0%, respectively). These results indicated that this method is useful to remove whole lipid droplets without losing mitochondria and improves cryotolerance of porcine GV oocytes. (c) 2005 Elsevier Inc. All rights reserved.
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