Journal
GENESIS
Volume 41, Issue 4, Pages 185-191Publisher
WILEY
DOI: 10.1002/gene.20113
Keywords
aristaless; Arx; BAC; Xenopus laevis; Xenopus; tropicalis; transgenesis; recombineering
Categories
Funding
- NEI NIH HHS [R01 EY012995-04, R01 EY012995, R01 EY012995-03, R01 EY012995-05] Funding Source: Medline
- NICHD NIH HHS [HD34615] Funding Source: Medline
Ask authors/readers for more resources
The aristaless-related homeobox (Arx) gene is expressed in a dynamic pattern in the developing vertebrate forebrain. We identified a bacterial artificial chromosome (BAC) containing the Xenopus tropicalis Arx gene and replaced a portion of the first coding exon with a green fluorescent protein (GFP) expression cassette by homologous recombination in bacteria (recombineering). Transgenic X. laevis embryos obtained by microinjecting the modified BAC expressed GFP in the developing forebrain in a pattern identical to that of the endogenous Arx gene. Thus, this BAC contains transcriptional regulatory elements necessary for regulating proper expression in transgenic frogs. This work demonstrates that transgenesis using recombineered BACs is a viable technique in Xenopus. (c) 2005 Wiley-Liss, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available