Comparison of two sequential media for culturing cleavage-stage embryos and blastocysts: embryo characteristics and clinical outcome

This study was undertaken to compare the outcome of day 3, day 5, and frozen-thawed embryo transfer cycles where embryo culture was undertaken using the G1.2-G2.2 versus GIII series sequential media. A total of 400 day 3, 73 day 5 and 126 frozen-thawed embryo transfer cycles were analysed. Treatment cycles were quasi randomized on the day of oocyte retrieval to embryo culture in G1.2-G2.2 versus GIII series sequential media. Randomization was undertaken according to alternating weekdays. Significantly more embryos were of grade 1 or 2 quality on day 3 in the GIII group (P < 0.05). Likewise, more embryos in the GIII group had eight blastomeres on day 3 (P < 0.05) and were able to hatch spontaneously (P < 0.05). For day 3 embryos transfer cycles, implantation and clinical pregnancy rates were 14.4 and 25.7 versus 37.8 and 50.3% in G1.2-G2.2 and GIII groups respectively (P < 0.05). For day 5 embryo transfer cycles, implantation rates were significantly higher (29 versus 45%, P < 0.05) in GIII group. There was a trend towards higher pregnancy rates, however, this did not reach statistical significance due to fewer cycles analysed in this group. In-vitro culture in GIII series sequential media yields better quality embryos that implant more efficiently, compared with culture in G1.2-G2.2 media.