Journal
EXPERIMENTAL PARASITOLOGY
Volume 109, Issue 4, Pages 241-251Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2004.12.011
Keywords
Entamoeba histolytica; Endoplasmic reticalum; Sec61 alpha subunit; abscess; antisense peptide nucleic acid; CP5, cysteine protease 5; endoplasmic reticulum; PNA, peptide nucleic acid
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The Sec61 alpha protein is defined as a highly conserved essential integral component of the endoplasmic reticulum in eukaryotic cells. We report a detailed immunolocalization of the Entamoeba histolytica homologue of the Sec61 alpha subunit (EhSec61 alpha), which shows an irregular pattern throughout the cell and is also found on the cell surface, its effective down-regulation by means of antisense peptide nucleic acids and its effects on cell proliferation, subcellular distribution of two virulence factors, and the ability of the trophozoites to cause liver abscess in hamsters. Although Sec61 alpha levels are specifically decreased in antisense PNA-treated trophozoites, which proliferate more slowly than the controls, mobilization of the cysteine protease 5 and amoebapore to the cell surface is not significantly impeded and the capacity to induce liver abscess in hamsters is largely unaffected. The implications of these findings are discussed in the context of the peculiar cell biology of E. histolytica. (c) 2005 Elsevier Inc. All rights reserved.
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