Journal
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
Volume 192, Issue 4, Pages 1044-1052Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.ajog.2005.01.031
Keywords
in utero transplantation; allogeneic; sheep; mesenchymal stem cells
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Objective: Mesenchymal stem cells (MSC) have multiorgan differentiation capacity, providing the potential for prenatal treatment of genetic disorders. We address the question if in utero transplantation of MSC results in short-term organ-specific engraftment in the fetal sheep. Study design: Sheep fetal liver-derived MSC selected by adherence culture (passage 1) were transplantated into the fetal peritoneal cavity with ultrasound-guidance (mean gestational age, 59 days). After 14 days recipient fetuses were analyzed by fluorescence-activated cell sorting (FACS), real-time polymerase chain reaction (PCR), and immunohistochemistry. Results: Fetuses (n = 11) were transplanted with 7.7 x 10(6) MSCs (mean). All surviving fetuses (n = 5) showed engraftment with mean levels of 3.2% (lung), 0.8% (spleen), 0.6% (liver, brain), 0.4% (bone marrow), 0.1% (blood, thymus), and < 0.1% (kidneys) by flow cytometry. Immunohistochemistry showed organ-specific distribution. Conclusion: In utero transplantation of allogeneic MSC results in low level, multiorgan engraftment at 14 days post transplant. This supports the potential of ill utero MSC transplantation for the treatment of nonhematopoietic genetic disorders of the fetus. (c) 2005 Elsevier Inc. All rights reserved.
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