Journal
PROTOPLASMA
Volume 225, Issue 1-2, Pages 15-22Publisher
SPRINGER WIEN
DOI: 10.1007/s00709-005-0089-3
Keywords
adhesion; calcium gel; egg-box conformation; Sinapis alba; meristem; pectin
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The nature of pectins ( acidic, methyl-, or acetyl-esterified) in the shoot meristem of Sinapis alba was assessed by immunocytochemistry with the 2F4 monoclonal antibody in light and electron microscopy. This antibody is specific for egg-boxes'' - the polygalacturonic acid conformation induced by calcium as described in Liners et al. ( Plant Physiol. 99: 1099 - 1104, 1992). Hardly any acidic pectin was detected in meristem walls; the pectins were largely methyl- esterified and esterified by acetyl groups and/or other esters. After in situ chemical or enzymatic de-esterification, labeling was distributed over the primary wall and the middle lamella of meristematic cells. Acidic pectin and Ca(2+)-cross-linked homo-galacturonans were absent from the pit fields, where plasmodesmata traverse the middle lamella. The type and distribution of pectins are discussed in relation to cellular adhesion between active meristem cells.
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