Journal
JOURNAL OF CELL SCIENCE
Volume 118, Issue 7, Pages 1537-1547Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.02285
Keywords
Listeria monocytogenes; cytoskeleton; HGF; actin; Rac; Cdc42; phagocytosis
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Funding
- NIGMS NIH HHS [GM58801] Funding Source: Medline
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Internalisation of the pathogenic bacterium Listeria monocytogenes involves interactions between the invasion protein InIB and the hepatocyte growth factor receptor, Met. Using colocalisation studies, dominant-negative constructs and small interfering RNA (siRNA), we demonstrate a cell-type-dependent requirement for various WASP-related proteins in Listeria entry and InIB-induced membrane ruffling. The WAVE2 isoform is essential for InIB-induced cytoskeletal rearrangements in Vero cells. In HeLa cells, WAVE1, WAVE2 and N-WASP cooperate to promote these processes. Abil, a key component of WAVE complexes, is recruited at the entry site in both cell types and its inactivation by RNA interference impairs InIB-mediated processes. Ena/VASP proteins also play a role in Listeria internalization, and their deregulation by sequestration or overexpression, modifies actin cups beneath entering particles. Taken together, these results identify the WAVE complex, N-WASP and Ena/VASP as key effectors of the Met signalling pathway and of Listeria entry and highlight the existence of redundant and/or cooperative functions among WASP-family members.
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