Journal
GLYCOBIOLOGY
Volume 15, Issue 4, Pages 323-333Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwi015
Keywords
core oligosaccharide; lipopolysaccharide; mass spectrometry; nuclear magnetic resonance; Pasteurella multocida
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Pasteurella multocida is an important multipecies veterinary pathogen. The cell surface lipopolysaccharide (LPS) is an important virulence factor and forms the basis of the serotyping scheme, although little structural information about it is known. The structure of the LPS from the Pasteurella multocida genome strain Pm70 was elucidated in this study. The LPS was subjected to a variety of degradative procedures. The structures of the purified products were established by monosaccharide and methylation analyses, NMR spectroscopy, and mass spectrometry. The structure of the core oligosaccharide was determined on the basis of the combined data from these experiments. Identification of the core oligosaccharide structure enabled a search for glycosyltransferase homologs in the Pm70 genome and revealed a clustering of the genes putatively responsible for outer core oligosaccharide biosynthesis.
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