Journal
ANALYTICAL BIOCHEMISTRY
Volume 339, Issue 1, Pages 83-93Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2004.12.031
Keywords
LC-MS/MS; translational frameshifting; frameshift site; frameshift motif; IS3; IS1372
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Translational frameshifting, a ubiquitous mechanism used to produce alternative proteins for different biological purposes, appears in a variety of genes in probably all organisms. In the past, the combinational use of sophisticated expression vectors, specific endopeptidases, and Edman degradation has been the main approach for identification of the translational frameshift sites. Although Edman degradation is highly reliable, it is also time-consuming and costly, In this article, we report a new liquid chromatography-tandem mass spectrometric (LC-MS/MS) approach for identifying the -1 translational frameshift sites. The approach consists of three steps: (i) LC-MS/MS analysis of the protein digests, (ii) primary data analysis using the known mRNA sequence, and (iii) advanced data analysis using a new database containing distinct mRNA sequences with single insertion at particular positions. We first validated our approach by analyzing the previously documented slippery sequence, A(4)G, from IS3. With this approach, we further determined whether the TTTTTTG (T(6)G) sequence of IS/372 from Streptomyces lividans had the -1 translational frameshifting potential. The identified amino acid sequence of the transframe peptide indicated that the -1 frameshifting occurred at the T(6)G motif, as predicted previously. The results on IS3 (A(4)G) and IS1372 (T(6)G) suggested that this approach is effective for the translational frameshifting studies. (c) 2005 Elsevier Inc. All rights reserved.
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