Journal
SENSORS
Volume 5, Issue 4-5, Pages 266-276Publisher
MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL
DOI: 10.3390/s5040266
Keywords
horseradish peroxidase; biosensor; hydrogen peroxide; chitosan
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An amperometric biosensor for hydrogen peroxide (H2O2) was developed via an easy and effective enzyme immobilization method with the sandwich configuration: ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basic electrode. In order to prevent the loss of immobilized HRP under optimized conditions, the biosensor surface was cross-linked with glyoxal. Ferrocene was selected and immobilized on the glassy carbon electrode surface as a mediator. The fabrication procedure was systematically optimized to improve the biosensor performance. The biosensor had a fast response of less than 10 s to H2O2, with a linear range of 3.5 x 10(-5) to 1.1 x 10(-3) M, and a detection limit of 8.0 x 10(-6) M based on S/N = 3.
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