Journal
ANALYTICA CHIMICA ACTA
Volume 534, Issue 1, Pages 63-67Publisher
ELSEVIER
DOI: 10.1016/j.aca.2004.03.079
Keywords
quantum dot; histidine tag; affinity tag; bioconjugation; single chain antibody; scFv; TNT; immunoassay
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We report a simple and versatile approach for the conjugation of luminescent CdSe-ZnS core-shell quantum dots (QDs) to proteins through coordination of engineered C-terminal oligohistidine sequences. Several histidine tail containing proteins were self-assembled onto the QD surface using this method. A recombinant antibody specific for the high explosive 2,4,6-trinitrotoluene (TNT) was conjugated to QDs through a carboxy terminal histidine tail and the bioconjugate used to detect TNT by competitive immunoassay. TNT was detected over the range of 10 mu g/ml down to 41 ng/ml using the scFv conjugated to QDs. These results open up the possibility to conjugate luminescent QDs to a whole range of proteins to form QD, bioconjugates that can be effectively used in bio-oriented applications, such as sensing, imaging, immunoassay and other diagnostics. (c) 2004 Elsevier B.V. All rights reserved.
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