Journal
ANALYTICA CHIMICA ACTA
Volume 534, Issue 2, Pages 199-205Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2004.11.051
Keywords
PNZ-Cl; ODAP; homoarginine; LC; polyamines; new extraction
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The paper proposes anew method to extract beta-N-oxalyl-(L)-alpha,beta-diaminopropionic acid (beta-ODAP), alpha-N-oxalyl-(L)-alpha,beta-diaminopropionic acid (alpha-ODAP), homoarginine and polyamines using 0.2 M HClO4 for only 1 h at 0-4 degrees C. Based on the proposed extraction, an assay is presented for simultaneously determining polyamines, the neurotoxin P-ODAP, its non-neurotoxic isomer alpha-ODAP and homoarginine in Lathyrus sativus extracts using a liquid chromatography (LC) system after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-Cl). The method exhibits some prominent advantages, which include: shorter extraction-time, simultaneous extraction of non-protein amino acids and polyamines and inhibition of the isomerization of beta-ODAP to alpha-ODAP. The assay proved satisfactory for the quantitative determination of L. sativus extracts of seeds and seedlings. (c) 2004 Elsevier B.V. All rights reserved.
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