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JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 280, Issue 14, Pages 14145-14153Publisher
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DOI: 10.1074/jbc.M500901200
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Peroxisome proliferator- activated receptor gamma( PPAR gamma) functions in various biological processes, including macrophage and adipocyte differentiation. Several natural lipid metabolites have been shown to activate PPAR gamma. Here, we report that some PPAR gamma ligands, including 15- deoxy- Delta(12,14)- prostaglandin J(2), covalently bind to a cysteine residue in the PPAR gamma ligand binding pocket through a Michael addition reaction by an alpha,beta-unsaturated ketone. Using rhodamine- maleimide as well as mass spectroscopy, we showed that the binding of these ligands is covalent and irreversible. Consistently, mutation at the cysteine residue abolished abilities of these ligands to activate PPAR gamma, but not of BRL49653, a non- covalent synthetic agonist, indicating that covalent binding of the alpha, beta- unsaturated ketone in the natural ligands was required for their transcriptional activities. Screening of lipid metabolites containing the alpha,beta- unsaturated ketone revealed that several other oxidized metabolites of hydroxyeicosatetraenoic acid, hydroxyeicosadecaenoic acid, and prostaglandins can also function as novel covalent ligands for PPAR gamma. We propose that PPAR gamma senses oxidation of fatty acids by recognizing such an alpha,beta- unsaturated ketone as a common moiety.
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