Journal
FEBS LETTERS
Volume 579, Issue 10, Pages 2161-2165Publisher
WILEY
DOI: 10.1016/j.febslet.2005.03.007
Keywords
cytidinediphosphate-diacylglycerol; cardiolipin synthase; functional expression; mitochondria; phospholipid synthesis; Arabidopsis thaliana
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Functional expression studies in microorganisms showed that the Arabidopsis thaliana gene At4g04870 represents the cardiolipin synthase (CLS) gene encoding a hydrophobic preprotein of 38 kDa with a cleavable signal peptide for the import into mitochondria. CLS of Arabidopsis over-expressed in Escherichia coli has an alkaline pH optimum, a strict requirement for divalent cations and a distinctly lower K. for cytidinediphosphate-diacylglycerol than for phosphatidylglycerol. It displayed a preference for both its substrates esterified with unsaturated acyl groups. Solubilization and purification experiments revealed that the protein requires a defined phospholipid environment, particularly the presence of cardiolipin, to acquire its catalytically active conformation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V.. All rights reserved.
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