Journal
BRITISH JOURNAL OF CANCER
Volume 92, Issue 7, Pages 1253-1260Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.bjc.6602499
Keywords
HER-2/neu; FISH; immunohistochemistry; HLA; immunotherapy
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The utilisation of antitumour T cells induced by cancer vaccination with HER-2 peptides or antibodies ( Herceptin) against HER-2, as immunotherapy for oesophageal cancer, is a novel and attractive approach. It is important to clarify the frequencies of HER-2 expression and gene amplification in patients with oesophageal squamous cell carcinoma (SCC) and to evaluate the relationship between HER-2 status and HLA haplotype, since the candidates for HER-2 peptide-based vaccination are restricted to a certain HLA haplotype. We determined the frequency of HER-2 expression using the HercepTest(TM) for immunohistochemistry and HER-2 gene amplification by fluorescence in situ hybridisation ( FISH) assay in oesophageal SCC ( n = 66). HER-2-positive tumours ( 1+/2+/3+) analysed by a HercepTest were observed in 30.3% of all the patients and HER-2 gene amplification evaluated by FISH was observed in 11.0% of all the patients, in which all HercepTest (3+) tumours were found to have gene amplification and three of six moderately positive (2+) tumours showed gene amplification. Furthermore, HER-2-positive cells were present more diffusely and were larger within each tumour in the patients who were HercepTest 3+ than those who were HercepTest 1+. Moreover, the survival rate in HER-2-positive group was significantly worse than that in HER-2-negative group. Also, the survival rate in the patients with HER-2 gene amplification was significantly worse than that without HER-2 gene amplification. In addition, oesophageal SCC patients with both HLA-A24-positive and HER-2-positive tumours ( 1+/2+/3+) accounted for 26% of these cases, and both HLA-A2- and HER-2-positive tumours accounted for 18% of them.
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