Journal
CURRENT BIOLOGY
Volume 15, Issue 7, Pages 650-655Publisher
CELL PRESS
DOI: 10.1016/j.cub.2005.02.029
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Hereditary spastic paraplegias (HSPs), a group of neurodegenerative disorders characterized by lower-extremity spasticity and weakness, are most commonly caused by mutations in the spastin gene, which encodes a AAA+ ATPase [1, 2] related to the microtubule-severing protein katanin [3]. A Drosophila homolog of spastin (D-spastin) was identified recently [4], and D-spastin RNAi-treated or genetic null flies show neurological defects, and protein overexpression decreases the density of cellular microtubules [5, 6]. Elucidating spastin's function and disease mechanism will require a more detailed understanding of its structure and biochemical mechanism. Here, we have investigated the effects of D-spastin, individual D-spastin domains, and D-spastin proteins bearing disease mutations on microtubuies in cellular and in vitro assays. We show that D-spastin, like katanin, displays ATPase activity and uses energy from ATP hydrolysis to sever and disassemble microtubules; disease mutations abolish or partially interfere with these activities.
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