Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 38, Issue 8, Pages 1080-1092Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2005.01.004
Keywords
ultrafine particles; oxidative stress; phospholipase A(2); lipid mediators; prostaglandin E-2; leukotriene B-4; 8-isoprostane; alveolar macrophages; free radicals
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In ambient aerosols, ultrafine particles (UFP) and their agglomerates are considered to be major factors contributing to adverse health effects. Reactivity of agglomerated UFP of elemental carbon (EC), Printex 90, Printex G, and diesel exhaust particles (DEP) was evaluated by the capacity of particles to oxidize methionine in a cell-free in vitro system for determination of their innate oxidative potential and by alveolar macrophages (AMs) to determine production of arachidonic acid (AA), including formation of prostaglandin E-2 (PGE(2)), leukotriene B-4 (LTB4), reactive oxygen species (ROS), and oxidative stress marker 8-isoprostane. EC exhibiting high oxidative potential induced generation of AA, PGE(2), LTB4, and 8-isoprostane in canine and human AMs. Printex 90, Printex G, and DEP, showing low oxidative capacity, still induced formation of AA and PGE(2), but not that of LTB4 or 8-isoprostane. Aging of EC lowered oxidative potential while still inducing production of AA and PGE(2) but not that of LTB4 and 8-isoprostane. Cellular ROS production was stimulated by all particles independent of oxidative potential. Particle-induced formation of AA metabolites and ROS was dependent on mitogen-activated protein kinase kinase 1 activation of cytosolic phospholipase A(2) (cPLA(2)) as shown by inhibitor studies. In conclusion, cPLA(2), PGE(2), and ROS formation was activated by all particle types, whereas LTB4 production and 8-isoprostane were strongly dependent on particles' oxidative potential. Physical and chemical parameters of particle surface correlated with oxidative potential and stimulation of AM PGE(2) and 8-isoprostane production. (c) 2005 Elsevier Inc. All rights reserved.
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