Journal
VACCINE
Volume 23, Issue 21, Pages 2791-2800Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2004.10.043
Keywords
East Coast fever; Theileria parva; baculovirus
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Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67 Delta SS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67 Delta SS and GP64:p67C induced antibodies with high ELISA titers that neutralized T parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100 mu g per dose, which is a major improvement over previous recombinant p67 products. (c) 2004 Elsevier Ltd. All rights reserved.
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